Etrasimod

Sphingosine-1-Phosphate Lyase Inhibition Alters the S1P Gradient and Ameliorates Crohn’s-Like Ileitis by Suppressing Thymocyte Maturation

Background: Lymphocytes recirculate from tissues to blood following the sphingosine-1-phosphate (S1P) gradient (low in tissues, high in blood), maintained by synthetic and degradative enzymes, among which the S1P lyase (SPL) irreversibly degrades S1P. The role of SPL in the intestine, both during homeostasis and IBD, is poorly understood. We hypothesized that modulation of tissue S1P levels might be advantageous over S1P receptor (S1PR) agonists (eg, fingolimod, ozanimod, etrasimod), as without S1PR engagement there might be less likelihood of potential off-target effects. Methods: First we examined SPL mRNA transcripts and SPL localization in tissues by quantitative reverse transcription polymerase chain reaction and immunohistochemistry. The in vivo effects of the SPL inhibitors 4-deoxypyridoxine hydrochloride (30 mg/L) and 2-acetyl-4 (tetrahydroxybutyl)imidazole (50 mg/L) were assessed through their oral administration to adult TNF∆ARE mice, which spontaneously develop Crohn’s-like chronic ileitis. The effect of SPL inhibition on circulating and tissue lymphocytes, transcriptional regulation of proinflammatory cytokines, and on the histological severity of ileitis was additionally examined. Tissue S1P levels were determined by liquid chromatography– mass spectrometry.

Mechanistically, the potential effects of high S1P tissue levels on intestinal leukocyte apoptosis were assessed via terminal deoxynucleotidyl transferase dUTP nick end-labeling assay and annexin 5 staining. Finally, we examined the ability of T cells to home to the intestine, along with the effects of SPL inhibition on cellular subsets within immune compartments via flow and mass cytometry. Results: S1P lyase was ubiquitously expressed. In the gut, immunohistochemistry predominantly localized it to small intestinal epithelia, al- though the lamina propria leukocyte fraction had higher mRNA transcripts. Inhibition of SPL markedly increased local intestinal S1P levels, induced peripheral lymphopenia, downregulated proinflammatory cytokines, and attenuated chronic ileitis in mice. SPL inhibition reduced T and myeloid cells in secondary lymphoid tissues and the intestine and decreased naïve T-cell recruitment. The anti-inflammatory activity of SPL inhibition was not mediated by leukocyte apoptosis, nor by interference with the homing of lymphocytes to the intestine, and was independent of its peripheral lymphopenic effect. However, SPL inhibition promoted thymic atrophy and depleted late immature T cells (CD4+CD8+ double positive), with accumulation of mature CD4+CD8- and CD4-CD8+ single-positive cells. Conclusions: Inhibition of the S1P lyase alters the S1P gradient and attenuates chronic ileitis via central immunosuppression. SPL inhibition could represent a potential way to tame an overactive immune response during IBD and other T-cell-mediated chronic inflammatory diseases.

INTRODUCTION
Interference with lymphocyte trafficking is a proven therapeutic strategy for inflammatory bowel disease (IBD),1 and antibodies against α4 integrins (natalizumab)2 and α4β7 (vedolizumab)3 are Food and Drug Administration approved for the treatment of Crohn’s and ulcerative colitis. Limitations of antibody-based therapies include high production cost, parenteral administration, risk of infection/cancer,4 and devel- opment of antidrug antibodies, with subsequent loss of clin- ical response (secondary failure). These have led to the testing of a newer class of orally administered small molecules that target sphingosine-1-phosphate (S1P) receptors (S1PR agon- ists), with the prototype drug (ie, fingolimod) approved for the treatment of multiple sclerosis.S1P is a bioactive sphingolipid that signals through 5G-protein-coupled receptors (GPCRs: S1PR1-5)5–7 and is a critical regulator of many pathophysiological processes (cell ad- hesion, migration, proliferation, differentiation, and survival).8 Ozanimod, a small molecule agonist that targets S1P receptors1 and 5, met all clinical trial end points in patients with mod- erate to severe ulcerative colitis (UC).9 Similarly, etrasimod, which targets S1P receptors 1, 4, and 5, has also shown pre- liminary efficacy and safety. S1PR agonists act as functional antagonists, which upon engagement polyubiquitinate10 re- ceptors, leading to their proteasomal degradation. This desen- sitizes lymphocytes, rendering them unable to sense S1P and recirculate.11S1P levels are tightly regulated by synthetic and deg- radative enzymes, which maintain low S1P levels in tissues through the action of the S1P lyase (SPL) and 2 phosphatases (Supplementary Fig. 1A).12 By contrast, S1P is high in blood, where SPL is not expressed. This difference constitutes the S1P gradient, which regulates lymphocyte recirculation and main- tains vascular integrity and lymphatic endothelial barrier func- tion.

However, as S1PRs signal and mediate numerous physiologic functions, we hypothesized that modulation of local S1P levels (without receptor engagement) might be ad- vantageous over S1PR agonists, which engage critical recep- tors, leading to potential off-target effects.15, 16Several inhibitors of the SPL have been described,17 par- ticularly, 2-acetyl-4 (tetrahydroxybutyl)imidazole (THI), which was found to be safe in phase 2 trials, and the vitamin B6 an- tagonist 4-deoxypyridoxine (DOP), which inhibits SPL in vivo (Supplementary Fig. 1B–D).7, 18 DOP attenuates vascular and immune defects in mouse sepsis19 and protects against collagen- induced arthritis and experimental autoimmune encephalomy- elitis.20–22 Yet, little is known regarding its effects on intestinal homeostasis or during IBD. Indeed, intestine-specific dele- tion of the enzyme actually hastens acute DSS-induced colitis through a yet unknown mechanism.23We examined the potential anti-inflammatory effects of SPL inhibition on a TNF-driven model of chronic ileitis (TNFΔARE) that recapitulates many features of Crohn’s di- sease.24, 25 First, we analyzed the expression of SPL on lympho- cytes via quantitative reverse transcription polymerase chain reaction (Q-PCR) and immunohistochemistry. Next, we inves- tigated the effect of in vivo SPL inhibition on peripheral and tissue immune cell subsets, on the S1P gradient, and on the severity of ileitis. Finally, we examined the underlying mech- anism of action of SPL inhibition on intestinal inflammation by studying its effects on the migration of T cells to the ter- minal ileum, on the induction of leukocyte apoptosis, and on thymocyte maturation.The B6.129S-Tnftm2Gkl/Jarn (TNF∆ARE) strain has been previously described.25 TNFΔARE mice were crossed with S1P1-eGFP+/+ reporter mice11 to generate TNFΔARE ΔARE/+/S1P1-eGFP+/+ mice. All mice used for these studies were hetero-zygous for the ∆ARE mutation. They were fed a standard dietwith or without pyridoxine supplementation (Dyets Inc., PA) ad libitum during in vivo studies. Animal procedures were ap- proved by the Institutional Animal Care and Use Committees of the University of California San Diego.Tissues were fixed, embedded, cut, and stained with he- matoxylin/eosin.

The severity of ileitis was assessed in a blinded fashion by a pathologist (P.J.) using a semiquantitative scoring system for murine ileitis, as previously described.26Cell IsolationCells were isolated from the thymus, mesenteric lymph nodes (MLNs), and spleen as previously described.21 Lamina propria leukocytes (LPLs) were isolated as described, with slight modification.27 Briefly, ilea were washed with cold phosphate- buffered saline (PBS) and cut into 2-cm pieces, incubated twice with 1X Hank’s balanced salt solution (HBSS) containing 1 mM of ethylene diamine tetraacetic acid (EDTA) for 15 minutes at room temperature, followed by digestion with 0.07 mg/mL of Liberase TL and 10 μg/mL of DNase I (Roche Diagnostics, Indianapolis, IN, USA) in RPMI 1640/10% FBS with contin- uous shaking at 37°C for 60 minutes. The mixture was passed through a 70-μm cell strainer to obtain a single-cell suspension.Cells from the indicated compartments were suspended in 1X PBS with 2% fetal bovine serum (FBS), supplemented with Fc block and stained with antimouse antibodies against CD4 (RM4-5), CD62L (MEL-14), CD44 (IM7), CD11b(M1/70; Biolegend, San Diego, CA, USA), CD8 (53–6.7), and B220 (RA3-6B2; eBioscience, San Diego, CA, USA) or corre- sponding isotype controls. Cells were washed twice and fixed in BD stabilizing fixative (BD Biosciences, San Jose, CA, USA). Flow cytometry acquisition was performed using a Cytek DxP10 (Cytek, Fremont, CA, USA). Data were analyzed using FLOWJO software (Tree Star Inc., Ashland, OR, USA).RNA Extraction, cDNA Synthesis, and Q-PCRTotal RNA was isolated using the RNeasy Kit (Qiagen, Valencia, CA, USA). RNA (500 ng) was reverse-transcribed with a high-capacity cDNA archive kit (Applied Biosystems, Foster City, CA, USA). Q-PCR assays for Sgpl1 (Mm00486079_ m1), Tnf (Mm00443258_m1), Il6 (Mm00446190_m1), Il12a (Mm00434169_m1), Ifng (Mm01168134_m1), Il4(Mm00445259_m1), Il17a (Mm00439618_m1), and Casp3 (Mm01195085_m1) were performed using the TaqMan Universal Master Mix (Applied Biosystems) with Gapdh as endogenous control.

Relative gene expression was calculated using the comparative CT(ΔΔCT) quantitation method withApplied BioSystems StepOne Software, version 2.3.Formalin-fixed, paraffin-embedded sections were deparaffinized and hydrated before antigen retrieval with citrate buffer, pH 6.0, in a prewarmed steamer (NESCO, Two Rivers, WI, USA) for 20 minutes. Sections were incubated for 10 min- utes with BLOXALL blocking solution (Vector Laboratories, Burlingame, CA, USA) to quench endogenous peroxidases, followed by incubation with sera to prevent nonspecific anti- body binding to Fc-receptors. Pretreatment of tissues with av- idin, followed by biotin, was done to block endogenous biotin binding sites (Vector Laboratories). For SPL detection, sections were immunostained with rabbit antimouse SPL polyclonal an- tibody (Bioss, Woburn, MA, USA) at a 1:200 dilution in PBT (0.5% BSA in PBS with 0.5% Tween-20) at 4°C for overnight. The sections were incubated with biotinylated antirabbit sec- ondary antibody (Vector Laboratories) at 1:1000 in PBT for 30 minutes at room temperature. Sections were incubated with the Elite ABC Kit (Vector Laboratories) for 30 minutes. Signal was detected with DAB (Vector Laboratories) for 2 minutes, and the slides were counterstained with hematoxylin.Mice with established inflammation were treated with DOP (30 mg/L), THI (50 mg/L) (Sigma-Aldrich, St. Louis, MO, USA), or vehicle control for 2 weeks, unless otherwise in- dicated. Mice were fed a standard diet without pyridoxine sup- plementation (Dyets Inc., Bethlehem, PA, USA).SPL activity was measured as per published protocol, with some modifications.28 Briefly, tissues were homogenized in tissue protein extraction reagent (T-PER) with protease in- hibitors (Halt Protease Inhibitor Cocktail, Thermo Fisher Scientific, Carlsbad, CA, USA), followed by centrifugation at ×500g for 5 minutes. Ten µL of reaction buffer (0.5 M of potassium phosphate 0.5M, PH 7.5, and 25 μM of sodium orthovanadate) and 10 µL of 125 mM S1P FS (SPL fluorogenic substrate, 1 mg, Cayman Chemical, Ann Arbor, MI, USA) were added to 75 µL of the lysate (25–30 μg) and incubated at 37°C for 6–12 hours. Fluorescence detection was performed at λex 325 nm and λem 420 nm in the presence or absence of 5 mMof semicarbazide (Sigma-Aldrich), a reactive compound thatinhibits SPL activity. The activity represents the semicarbazide sensitivity portion of the total activity.S1P was extracted from 200 μL of mouse plasma or tissue homogenate by adding 1 mL of 50/50 dichloromethane/ methanol, followed by vortexing for 10 seconds.

Samples were spun at 3000 rpm for 5 minutes, and the supernatant was re- covered. C17-S1P was used as an internal standard. The anal- ysis of S1P and sphingosine was carried out using liquidchromatography–mass spectrometry (LC-MS) as described previously.29Terminal Deoxynucleotidyl Transferase dUTP Nick End-Labeling AssayTo analyze apoptotic nuclei, 10 μm OCT frozen sections of ileum were prepared and stained according to manufacturer protocol (TACS TdT in situ, Fluorescein, 4812-30-K, R&D systems).T cells from spleen and MLNs of TNFΔARE mice were sorted using Pan T cell isolation Kit II (Miltenyi Biotec, Auburn, CA, USA) and stained with 3 μM carboxyfluorescein succinimidyl ester (Vybrant CFDA SE Cell Tracer Kit, Thermo Fisher Scientific, Carlsbad, CA, USA) according to the manufacturer’s instructions. Twelve million cells were injected intravenously to mice that were pretreated with DOP or ve- hicle. Twelve to 24 hours later, recipient mice were killed for fluorescently labeled cell quantification of lymphocyte homing.Antibody conjugation and staining protocols were obtained from the flow cytometry core at La Jolla Institute for Allergy and Immunology (LJI). Purified antibodies were con- jugated with the indicated metals for mass cytometry analysis using the MaxPAR antibody conjugation kit (Fluidigm, San Francisco, CA, USA) according to the manufacturer’s instruc- tions. A list of antibodies used for mass cytometry analysis can be found in Supplementary Table 1. Briefly, cells were suspended in 1 mL of cell staining buffer (CSB: 1X CyPBS containing 2 mM of EDTA, 0.1% BSA and 0.05% NaN3) at 1×107 cells mL−1, and cisplatin (DVS Sciences, Cell-ID Cisplatin, 201064) was added at a final concentration of 5 μM for 5 minutes at RT. Cells were stained with antibodies against surface markers for 30 min- utes at 4°C and fixed with 2% paraformaldehyde for overnight. Cells were permeabilized and stained with antibodies against intracellular (nuclear proteins) markers and 125-nM DNA intercalator for 30 minutes at room temperature; 1:10th of EQ beads (EQ Four Element Calibration Beads, 201078, Fluidigm) were added to samples, and data were acquired on cytometry by time of flight (CyTOF) Helios mass cytometer (LJI).

Computational analyses of mass cytometry data were done with the PhenoGraph algorithm in cytofkit,30 using R studio, version 1.0.153 (http://www.r-project.org/), and the cytofkit package, release 3.6, downloaded from Bioconductor (https:// www. bioconductor.org/packages/release/bioc/html/cytofkit. html), opened in R. Normalized and manually gated singlet (191Iridium (Ir) + 193Ir +) viable (195Platinum (Pt) +) events or singlet (191Ir + 193Ir +) viable (195Pt +) CD3+ MHCII- events were imported into cytofkit, subjected to PhenoGraph analysis, and clustered based on all antibodies (for singlet viable events),or on all markers excluding parameters gated on for CD3+ MHCII- events (for singlet viable CD3+ MHCII- events), using the following settings: merge method: “ceil” (60,000 events total for live singlet events) or “all” for CD3+ MHCII- events; trans- formation: cytofAsinh; cluster method: Rphenograph; visuali- zation method: tSNE; and cellular progression: NULL. Results were visualized via the R package “Shiny,” where labels, dot size, and cluster color were customized. Clusters were colored according to phenotype based on the median expression of var- ious markers. The frequency of each cluster and cellular pheno- type was determined via .csv files generated by the algorithm. All statistical tests were performed and graphs were generated using GraphPad Prism 7.Statistical AnalysisResults are expressed as mean ± SEM unless other- wise indicated. Data were analyzed using GraphPad Prism 7 (GraphPad Software, Inc., San Diego, CA USA). Significant differences between individual groups were calculated using the 2-tailed unpaired t test.

RESULTS
To investigate the tissue and cellular distribution of SPL, we assessed mRNA transcripts in indicated tissues (Fig. 1A). We noticed that within the gastrointestinal tract, SPL expres- sion was highest in the small bowel, the primary site of in- flammation in TNFΔARE mice. SPL mRNA expression was assessed in whole ileal tissue and from fractionated epithelial and LPL fractions. SPL expression was highest on the leu- kocyte fraction, followed by the epithelial fraction (Fig. 1B). Plasma S1P levels were higher than tissue levels (ileum, MLNs, and thymus) (Fig. 1C). Immunohistochemistry localized the ex- pression of SPL to the thymus, MLNs, spleen, and ileum (Fig. 1D). The negative control lacked the primary anti-SPL anti- body. Within the thymus, there was higher expression in the me- dulla compared with the cortex. Within MLNs, SPL expression localized to the deep cortical T-cell zone, whereas in the spleen, SPL was expressed on white pulp nodules, along with red pulp matrix. Interestingly, in the inflamed intestine, SPL signal local- ized prominently to the epithelium, intraepithelial region, and intestinal crypts, consistent with previous observations.23, 31S1P Lyase Inhibition Increased Local Tissue S1P Levels, Induced Peripheral Lymphopenia,Downregulated Proinflammatory Cytokines, and Ameliorated Ileitis in TNFΔARE MiceMice were administered the SPL inhibitor DOP, which competes with pyridoxal 5′-phosphate (PLP) coenzyme, along with a vitamin B6 (pyridoxine)–deficient diet for 2 weeks. Aftertreatment, the S1P gradient had been obliterated and S1P levels in the ileum, MLNs, and thymus were similar to plasma levels (*P < 0.05; *P < 0.05; ***P < 0.001) (Fig. 2A) in treated mice compared with controls. DOP additionally induced peripheral lymphopenia, with a lower frequency and number of blood CD4+ and CD8+ T cells (***P < 0.001) compared with controls. By contrast, the frequency and number of B220+ cells were un- affected (Fig. 2B). DOP additionally reduced the ileal mRNA transcripts of TNF, IL-6 (**P < 0.01), IL-12 (*P < 0.05), IFN-γ, and IL-17 (**P < 0.01) compared with controls (Fig. 2C). DOP also attenuated active (granulocytic) inflammation (6.9 ± 1 vs 2.3 ± 2), chronic (lymphocytic/monocytic) inflammation (6.9 ±1.3 vs 3 ± 1.8), villus distortion (6.6 ± 1.3 vs 3.5 ± 1.4), and the total inflammatory indices (sum of individual indices: 20.5± 3.1 vs 8.8 ± 4.7; ***P < 0.001) compared with controls (Fig. 2D). In addition, histological hallmarks of ileitis, such as leu- kocyte infiltration and hypertrophy of the muscularis propria, were noticeably different (Fig. 2E). DOP significantly inhibited SPL activity (*P < 0.05) in the ileum without affecting SPL mRNA transcripts (Supplementary Fig. 1C, D). A pyridoxine- deficient diet alone did not induce lymphopenia or affect the se- verity of ileitis, compared with a standard diet (Supplementary Fig. 2A–C). In DOP-treated mice, the lymphopenic effect was greater on circulating naïve CD4+ and CD8+ T cells (***P < 0.001); thereby, the percentage of effector T cells increased (***P < 0.001) in the DOP-treated group compared with con- trols (Supplementary Fig. 3A, B). In addition, the median fluo- rescence intensity (MFI) of S1PR1 on CD4+, CD8+ T (**P < 0.01), and B220+ (*P < 0.05) cells was lower in the DOP-treated group compared with controls (Supplementary Fig. 3C, D). We observed a lymphopenic effect on circulating T cells as early as 24 hours after DOP treatment (data not shown). However, although DOP treatment for 1 week significantly reduced cir- culating T cells, it did not affect ileitis severity (Supplementary Fig. 4A–C). Taken together, these results demonstrate that SPL inhibition abolishes the S1P gradient, potentially altering cell recruitment and/or egress to/from the intestine. SPL inhibition predominantly decreased naïve CD4+ T-cell and CD8+ T-cell subsets from circulation, downregulated S1PR1 surface ex- pression on lymphocytes, decreased proinflammatory cytokine transcripts in the intestine, and attenuated chronic ileitis.We then assessed the effects of DOP treatment for 2 weeks on lymphocytes and monocytes within relevant immune compartments (ie, spleen, MLNs, and ileal LP). Total cell counts were uniformly reduced in the spleen (****P < 0.0001), MLNs (**P < 0.01), and ileal LP (*P < 0.05) after DOP, compared with controls (Fig. 3A–C). However, SPL inhibi- tion differentially affected lymphocyte subsets with reducedCD4+ and CD8+ T cells (****P < 0.0001), but without an ef- fect on B cells in any of these 3 compartments (Fig. 3A–C). In the ileal lamina propria, we observed a modest decrease in the frequency of CD8+ T cells (**P < 0.01) and myeloid cells (CD11b+; *P < 0.05) (Fig. 3C). Further subset analyses identified the most noticeable effects on splenic naïve CD4+ and CD8+ T cells after DOP treatment (Fig. 3A–C), yet the total cell counts were markedly decreased in all compartments assessed.Deep Immunophenotyping of Leukocyte Subsets After S1P Lyase Inhibition in TNFΔARE MiceWe next investigated the breadth of the effect of DOP on leukocyte subsets via high-dimension mass cytometry (CyTOF) analysis on a small number of DOP and vehicle- treated mice (n = 3/treatment). Cells from blood, MLNs, and ileal LP were stained with a panel of 34 metal-conjugatedantibodies (Supplementary Table 1), acquired using a Helios 2 mass cytometer and analyzed using the PhenoGraph al- gorithm. In peripheral blood, DOP treatment resulted in a pronounced decrease in both CD4+ and CD8+ T cells, con- sistent with findings observed via flow in the spleen, with an intermediate decrease in the frequency of CD19+ B cells and a significantly increased frequency of CD11b+ myeloid cells (Supplemental Fig. 5A, B), different from what was observed in the spleen via flow cytometry. Cytof similarly showed that DOP treatment decreased naïve CD4+ and CD8+ T cells, with a trend toward increased frequency of CD44high effector CD4+ and CD8+ T cells (Supplemental Fig. 5C–E). In contrast, compared with peripheral blood, the distribution of leukocytesubsets showed only minimal alterations in MLNs and ileal LP after DOP treatment, despite the major effects on the cell counts observed by flow cytometry in the MLNs and ileum (Fig. 3A–C). In the MLNs, there was no overall change in the relative frequencies of CD4+ and CD8+ T cells, CD11b+ my- eloid cells, or CD19+ B cells, with a modest decrease in central memory CD4+ T cells and a modest increase in the frequency of CD44high effector CD8+ T cells (Supplemental Fig. 5F–J). Similarly, in ileal LP, DOP treatment was associated with a modest decrease in the frequency of CD8+ T cells and trends toward decreased frequency of CD11b+ myeloid cells and of CD19+ B cells. Ileal LP T-cell subsets showed no statistically significant changes in the cell percentages after DOP treatment(Supplemental Fig. 5K–O). These high-dimensional data cor- roborate overall trends observed with the flow cytometry– based studies (major percentage shifts observed in blood/ spleen) but not in the MLNs and ileum, where DOP similarly decreases all leukocyte types, without singularly affecting spe- cific cell subsets.The Lymphopenic Effect of 2-acetyl-4 (tetrahydroxybutyl)Imidazole Is Independent of its Anti-inflammatory ActivityThe safety of the SPL inhibitor THI has been tested in patients with rheumatoid arthritis.18, 20 To assess its potential anti-inflammatory effects on ileitis, 10-week-old TNFΔARE mice were administered THI in drinking water for 2 and 4 weeks. THI induced peripheral lymphopenia of CD4+ T cells(**P < 0.01) and CD8+ T cells (***P < 0.001), but like DOP, it had no significant effect on peripheral B220+ cells compared with vehicle-treated controls (Fig. 4A). Also, different from DOP, histopathological analysis did not show an anti-inflam- matory effect in mice treated for 2 or 4 weeks (Fig. 4B). To further understand the lack of a therapeutic effect of THI, we assessed S1P levels and found no significant changes in the ileum, MLNs, or plasma S1P in THI-treated mice compared with controls (Fig. 4C). Similarly, THI had no effect on overall S1PR1 expression (MFI) on CD4/8 or B220+ lymphocytes (Fig. 4D, E). These results suggests that the lymphopenic ef- fect of THI is independent of its anti-inflammatory activity on ileitis and that the mechanism of action of these drugs is not solely predicated on its ability to induce peripheral lymphopenia.Given the known effects of S1P on tightening the lymphatic endothelial barrier, we hypothesized that elevated local S1P levels could similarly enhance the endothelial barrier at postcapillary venules and impede leukocyte transmigration and recruitment to the intestine.13, 32–35 To test this hypothesis, we isolated and labeled negatively-selected T cells with CFSE (after depleting B cells) and injected them into 10-week-old TNFΔARE mice that had received DOP or vehicle for 48 hours before transfer. Cells wereallowed to traffic for 24 hours to quantify the number of cells able to home to different compartments via flow cytometry. Analysis showed a significant (**P < 0.01) accumulation of CFSE+ cells in the spleen of recipient mice treated with DOP compared with vehicle controls, but no effect on traffic to the thymus, MLNs, or ileal LP (Fig. 5). Subset analysis revealed a preponderance of naïve CD4+ T cells retained in the spleen (Fig. 5). Thus, the an- ti-inflammatory effect of DOP is not mediated by interference with de novo recruitment of T cells to the intestine, as has been argued for anti-integrin therapies. Elevated S1P levels result in accumulation of ceramide, a lipid upstream of S1P, which is known to induce T-cell apop- tosis.36 We hypothesized that elevated S1P levels should increase all upstream metabolites, leading to increased local ceramide levels and leukocyte apoptosis in the intestine. However, we did not observe changes in the percentage of apoptotic CD4, CD8, or total cells via annexin V staining in cells from the ileal LP of mice treated with DOP for 2 days, 1 week, or 2 weeks (Fig. 6A). Caspase 3 levels were unchanged despite a dramatic in- crease in sphingosine upstream of S1P (Fig. 6B, C). Similarly, terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) analysis did not show significant changes in thenumber of apoptotic nucleated cells in the ileal crypts or villi of DOP-treated mice compared with controls (Fig. 6D). These results suggest that the anti-inflammatory effects of SPL inhibi- tion are not mediated by the induction of leukocyte apoptosis by ceramide in the chronically inflamed intestine of TNFΔARE mice.S1P Lyase Inhibition Promoted Thymic Involution and Cortical Atrophy by Depletion of Stage II CD4+/CD8+ (Double-Positive) T Cells in TNFΔARE MiceFinally, we examined whether the anti-inflammatory ef- fect could be due to central immunosuppression. Thymicsize, weight, and cell counts (***P < 0.001) were markedly re- duced in DOP-treated mice compared with controls (Fig. 7A). Reduction in thymic size was progressive with DOP administra- tion over 2, 4, 6, 8, 10, and 14 days (Supplementary Fig. 6). In the DOP-treated group, the cortical region showed atrophy over time (data not shown), with marked atrophy by day 14, as evi- denced by near absence of corticomedullary demarcation (Fig. 7B, C). The percentage of CD4+C8+ double-positive thymo- cytes in the DOP group progressively decreased, compared with controls (1% ± 1% vs 69% ± 16% at day 14). This decrease inCD4+CD8+ double-positive thymocytes occurred in parallel with a relative increase in CD4+CD8- and CD4-CD8+ single- positive thymocytes over time. On day 14, most of the cells left in the thymus were CD4+CD8- (55% ± 5% vs 15% ± 6%) andCD4-CD8+ (37% ± 3% vs 7% ± 4%) single-positive cells, com- pared with controls (Fig. 7D). These results suggest that thymic involution and interference with T-cell maturation play a major role on the anti-inflammatory effect of SPL inhibition. DISCUSSION Drugs that target the S1P axis are rapidly advancing through the IBD clinical trial pipeline.38 Fingolimod has been approved for the treatment of multiple sclerosis, ozanimod is currently on phase 3 trials both for UC and CD, and etrasimod has recently completed phase 2 in UC.In previous studies, we observed that the S1P-degrading enzymes (S1P phosphatases and SPL) were downregulated in the ilea of IBD mouse models and in patients with IBD,40 which could potentially represent a retention signal that impedes the recirculation of lymphocytes, contributing to their accumula- tion in inflamed tissues over time.41, 42 Studies by Schwab and Cyster7 showed that lymphocyte egress from lymphoid tissue is mediated by S1P gradients through the activity of SPL. SPL inhibition increased S1P levels in lymphoid tissues such as the thymus, spleen, and lymph nodes and downregulated S1PR1 expression on lymphocytes. However, the role of SPL during intestinal homeostasis and chronic intestinal inflammation had not been examined. Indeed, in a prior study, acute DSS co- litis was aggravated by SPL inhibition.23 In the present study, we show that SPL is ubiquitously expressed; however, within the intestine, SPL is predominantly localized to the epithe- lium and infiltrating leukocytes, predominantly of the small intestine. As the small intestine is the main intestinal segment affected in TNFΔARE mice, this model of IBD might be of particular translational relevance. Here, we demonstrate that local S1P levels can be manipulated for therapeutic purposes during experimental IBD. S1P levels are high in plasma and low in the ileum, MLNs, and thymus of uninflamed controls and inflamed TNFΔARE mice. S1P levels were higher in the plasma of inflamed mice, compared with those of uninflamed mice, which may partially explain increased recirculation of im- mune cells from the inflamed intestine. We further demonstrate that although SPL inhibition did not alter S1P levels in plasma, it markedly increased S1P tissue levels from about 1 picomol/ mg to 100, 400, and 2000 picomol/mg in the MLNs, thymus, and ileum of TNFΔARE mice, respectively. SPL inhibition in- duced peripheral lymphopenia and downregulated S1PR1 ex- pression (desensitization) on circulating lymphocytes, as shown previously in healthy mice.7 Yet in the present study, we show a major therapeutic effect on ileitis, with a marked decrease of inflammatory cells in the spleen, MLNs, and ileum. There was additionally reversal of villus atrophy and crypt and muscularis hypertrophy along with downregulation of proinflammatory cytokine transcripts (ie, TNF, IL-6, IL-12, IFN-γ, and IL-17) in the ileum of mice with chronic ileitis. Although the SPL in- hibitor THI7, 18, 31 induced significant lymphopenia, it did not ameliorate ileitis after 2- or 4-week administration, suggesting that the anti-inflammatory effects of these compounds might be independent of their ability to deplete peripheral T cells. During IBD, there is dysregulated recruitment of leuko- cytes in response to elevated chemoattractants and enhanced expression of integrin ligands (eg, MAdCAM-1, VCAM-1), which contribute to the pathogenesis of the disease.43, 44 The proposed mechanism of action of antibodies against integrins α4β1/α4β7 (ie, natalizumab), α4β7 (ie, vedolizumab), and β7 (ie, etrolizumab) is interference with the binding of integrins on the lymphocyte surface with their respective endothelial ligands (ie, VCAM-1, MAdCAM-1) at postcapillary venules. By con- trast, S1P receptor modulators are believed to act by blocking lymphocyte egress from secondary lymphoid organs. Although we considered that SPL inhibitors may exert their anti-in- flammatory activity by tightening the endothelial barrier and decreasing recruitment at postcapillary venules, we found that SPL inhibition did not affect de novo recruitment of CD4+ T cells to the MLNs or intestine.45 Yet DOP markedly decreased the overall inflammatory infiltrate in all relevant sites (LP, MLNs, and spleen).46 However, we found a profound reshaping of the lymphocyte-to-myeloid ratio in peripheral blood after DOP treatment. The high-dimensional approach emphasized the compartment-specific effects of SPL inhibition on leuko- cyte distribution (predominantly observed in peripheral blood, followed by spleen), whereas in the MLNs and ileum we ob- served an overall depletion of cell counts of most cell types, without major effects on specific cellular subsets. Many immunosuppressive drugs in current clinical use are believed to work in part by inducing apoptosis of inflam- matory cells within the lamina propria.47 Ceramide, a known proapoptotic molecule, may potentially mediate this effect after SPL inhibition.36 Although we did not directly measure ceramide, there was marked elevation of sphingosine levels in DOP-treated mice. As ceramide is 1 step upstream of sphingo- sine, our findings suggest that ceramide levels may be similarly increased.48 However, there were no significant changes in the number of apoptotic cells in the ileum of TNFΔARE mice at any time point (early or late), either by annexin V staining or by TUNEL assay. Thus, it is unlikely that the anti-inflammatory properties are mediated by induction of leukocyte apoptosis within the intestinal lamina propria. By contrast, in the thymus DOP had a remarkable ef- fect on thymic mass and cell counts, similar to what has been reported after long-term administration of S1PR agonist fingolimod in rodents.47, 49 DOP treatment induced marked thymus involution, with disappearance of the cortical region and near absence of immature CD4+C8+ double-positive thymocytes, along with a relative increase in mature CD4+CD8- and CD4-CD8+ single-positive thymocytes. SPL was expressed predominantly in the medulla. Its action of degrading S1P al- lows for S1PR1 re-expression (resensitization) and egress on mature thymocytes by maintaining low thymic S1P levels.50 However, lyase inhibitors induced mature thymocytes to accu- mulate in the thymic medulla, where they may eventually un- dergo apoptosis. Our findings support the hypothesis that the anti-inflammatory effects of SPL inhibition on chronic ileitis are mediated by central immune suppression and interference with thymocyte maturation. In summary, our studies demonstrate the remarkable an- ti-inflammatory activity of SPL inhibition in a chronic model of Crohn’s-like ileitis after obliteration of the S1P gradient. SPL inhibition downregulated proinflammatory cytokines, re- duced inflammatory infiltrates, and restored intestinal archi- tecture. Based on our findings, we hypothesize Etrasimod that short-term treatment with these agents may modulate overactive T-cell immune responses, as seen in IBD and other T-cell-mediated diseases. Whether this strategy might be too immunosuppres- sive in humans and result in serious opportunistic infections will likely depend on the timing of recovery and restoration of lymphocyte counts after discontinuation of therapy.